Procalcitonin (PCT)
Name | Anti-Human Procalcitonin (PCT) antibody | ||
Catalog # | R58w3 | R150e6 | R151h2 |
Platforms Pairs |
Chemiluminescence immunoassay (CLIA) | Immunochromatography assay (ICA) | Turbidimetric Immunoassay (TIA) |
R58w3 (Capture) -R150e6 (Detection) R151h2 (Capture) -R150e6 (Detection) |
R150e6 (Capture) - R151h2 (Detection) | R150e6 R151h2 |
|
Description | Mouse monoclonal antibody, cultured in vitro | ||
Buffer | 1xPBS | ||
Purity | Purity>98%, purified by Protein A/G chromatography | ||
Storage | Aliquot and store at -20°C or lower. Avoid freeze / thaw cycles. |
PCT Antibody Conjugated Magnetic Beads
Name | Catalog # |
PCT antibody conjugated magnetic beads | B123l1 |
B124l1 | |
B125l1 |
Labeled PCT Antibody
Name | Catalog # |
Biotin labeled PCT antibody | L126l1 |
Acridinium ester (AE) labeled PCT antibody | L120l1 |
Alkaline phosphatase (ALP) labeled PCT antibody | L122l1 |
Name | Procalcitonin (PCT) antigen |
Description | Recombinant, C-terminal His-tagged, in vitro expressed from E. coli |
Applications | Calibrator and quality control product |
Catalog # | P1103 |
Purity | >90%, analyzed by R250-stained SDS-PAGE |
Buffer | 1 x PBS,pH 7.4 |
Storage | Aliquot and store at -80°C. Avoid freeze / thaw cycles. |
SDS-PAGE |
Predicted MW around 13.7 kDa |
Sample No. | Concentration of Samples (ng/mL) |
Concentration of Detection (ng/mL) |
1 | 0.018 | 0.015 |
2 | 0.033 | 0.028 |
3 | 0.016 | 0.018 |
... | ... | ... |
112 | 27.584 | 28.145 |
113 | 46.150 | 46.200 |
Fig.1 Correlation of PCT clinical samples on ALP-CLIA platform
Table.1 Data of clinical comparative analysis
Sample No. | Concentration of Samples (ng/mL) |
Concentration of Detection (ng/mL) |
1 | 0.16 | 0.15 |
2 | 0.29 | 0.30 |
3 | 0.43 | 0.42 |
... | ... | ... |
29 | 56.28 | 62.51 |
30 | 68.64 | 58.72 |
Fig.2 Coincidence rate of PCT clinical samples on TRFIA platform
Table.2 Data of clinical comparative analysis
<0.05 ng/ml | Normal |
<0.5ng/ml | No or slight systemic inflammation |
0.5-2ng/ml | Medium level of systemic inflammation |
2-10ng/ml | Suspected sepsis |
≥10ng/ml | Critical bacterial sepsis |
References
[1] Becker KL, Nylén E S, WhiteJ C, et al. Clinical review 167: Procalcitonin and the calcitonin gene family of peptides in inflammation, infection, and sepsis: a joumey from calcitonin back to its precursors.[J]. Journal of Clinical Endocrinology & Metabolism, 2004, 89(4): 1512.
[2] Weglohner W R D, StruckJ S c, Morgenthaler N, et al. Isolation and characterization of serum procalcitonin from patients with sepsis.[J]. Peptides, 2001, 22(12): 2099-103.
[3] Müller B, White J C, NylénE s, et al. Ubiquitous expression of the calcitonin-i gene in multiple tissues in response to sepsis[J]. Journal of Clinical Endocrinology & Metabolism, 2001, 86(1): 396.
[4] Levin A S. Overcoming Antimicrobial Resistance[J]. Brazilian Journal of Infectious Diseases, 2008, 12: 10-21.
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